FIG 8.

Absence of PML reduces replication compartment formation. HFF cells were cultivated on coverslips and treated with siRNAs specific for PML or with nontargeting siRNA. Cells were infected with 7134 virus at an MOI of 5 or mock infected and then were fixed at 6 hpi. Immunofluorescence staining was performed with antibodies specific for PML and ICP8. (A) Images showing nuclei of representative mock-infected cells in the presence or absence of PML. Channels specifically detected PML (green) or ICP8 (red), and merged images are also shown. (B) Same as panel A, but with 7134 virus infection. (C) Cells showing ICP8 expression above the background were sorted into two groups according to the morphology of staining (diffuse versus replication compartment). Percentages of cells with diffuse or replication compartment-like ICP8 staining are depicted for both siNT and siPML conditions. (D) Percentages of cells showing ICP8 staining above the background for either the siNT or siPML background.