FIG 5.

SB258585 does not alter surface localization of the other main HCV entry factors. (A) Huh-7 cells were treated for 2 h with DMSO or increasing concentrations of SB258585. Surface biotinylation followed by biotin immunoprecipitation was performed, and a representative Western blot for OCLN is shown. The quantity of biotinylated OCLN was determined from Western blots by use of Fiji (n = 3). (B to E) Huh-7 cells were treated for 2 h with DMSO or increasing concentrations of SB258585. CD81 (B), EGFR (C), SRB1 (D), and E-cadherin (E) expression levels were analyzed by flow cytometry. Curves from a representative experiment are shown. Mean fluorescence intensities relative to those for the DMSO-treated condition are presented. All results are presented as means ± SEM (n = 3). One-way ANOVA followed by the Dunnett posttest was performed for statistical analysis. ns, nonsignificant.