TABLE 1.
Genetic and phenotypic characteristics of the carbapenemase-encoding Enterobacteriaceae (n = 41)
| Bacterial isolate |
MLST | Groupa | Carbapenemase-encoding genetic structure |
||||||
|---|---|---|---|---|---|---|---|---|---|
| No. | Strain ID | Taxonomy | Gene | Plasmid structureb | Replicon type | Size (bp) | GenBank accession no. | ||
| 1 | 947385799 | E. coli | ST167 | blaNDM-5 | pNDM-MGR194-like | IncX3 | 46,253 | NC_022740.1 | |
| 2 | 944535499 | K. pneumoniae | ST14 | blaOXA-232 | PittNDM01 plasmid4 | ColPK3 | 6,141 | NZ_CP006802.1 | |
| 3 | 939996824 | K. pneumoniae | ST101 | I | blaGES-5 | pCHE-A1 | IncQ | 8,201 | KX244760.1 |
| 4 | 945154233 | K. pneumoniae | ST101 | I | blaGES-5 | pCHE-A1 | |||
| 5 | 945165838 | K. pneumoniae | ST101 | I | blaGES-5 | pCHE-A1 | |||
| 6 | 945169659 | K. pneumoniae | ST101 | I | blaGES-5 | pCHE-A1 | |||
| 7 | 957083320 | K. pneumoniae | ST101 | I | blaGES-5 | pCHE-A1-like | |||
| 8 | U44822 | K. pneumoniae | ST101 | I | blaGES-5 | pCHE-A1 | |||
| 9 | 957083896 | K. pneumoniae | ST101 | I | blaGES-5 | pCHE-A1-like | |||
| 10 | 957089165 | K. pneumoniae | ST101 | I | blaGES-5 | pCHE-A1 | |||
| 11 | 960186733 | K. pneumoniae | ST101 | II | blaNDM-1 | p19-10_01 | IncFIB(K) | 223,434 | CP023488.1 |
| 12 | 950171785 | K. pneumoniae | ST101 | II | blaNDM-1 | p19-10_01-like | |||
| 13 | 950173000 | K. pneumoniae | ST101 | II | blaNDM-1 | p19-10_01-like | |||
| 14 | 951373950 | K. pneumoniae | ST101 | II | blaNDM-1 | p19-10_01-like | |||
| 15 | 951362657 | K. pneumoniae | ST2016 | II | blaNDM-1 | p19-10_01-like | |||
| 16 | 951384356 | K. pneumoniae | ST101 | II | blaNDM-1 | p19-10_01-like | |||
| 17 | 951363981 | K. pneumoniae | ST101 | II | blaNDM-1 | p19-10_01-like | |||
| 18 | 950142398 | K. pneumoniae | ST2017 | III | blaNDM-1 | p18-43_01 | IncR_1, IncFIB(pB171), IncFII(Yp) | 212,326 | CP023554.1 |
| 19 | 941530379 | K. pneumoniae | ST323 | blaNDM-1 | p18-43_01-like | ||||
| 20 | 950117510 | K. pneumoniae | ST2017 | III | blaNDM-1 | p18-43_01-like | |||
| 21 | 950118422 | K. pneumoniae | ST2017 | III | blaNDM-1 | p18-43_01-like | |||
| 23 | 939742031 | K. michiganensis | ST170 | blaNDM-1 | p18-43_01-like | ||||
| 24 | 950005607 | S. marcescens | NAc | 1 | blaNDM-1 | p18-43_01-like | |||
| 25 | 950196656 | S. marcescens | NA | 1 | blaNDM-1 | p18-43_01-like | |||
| 26 | 950163360 | S. marcescens | NA | 1 | blaNDM-1 | p18-43_01-like | |||
| 27 | 950164094 | S. marcescens | NA | 1 | blaNDM-1 | p18-43_01-like | |||
| 28 | 950165859 | S. marcescens | NA | 1 | blaNDM-1 | p18-43_01-like | |||
| 29 | 950166381 | S. marcescens | NA | 1 | blaNDM-1 | p18-43_01-like | |||
| 30 | 950174583 | S. marcescens | NA | 1 | blaNDM-1 | p18-43_01-like | |||
| 31 | 950172946 | S. marcescens | NA | 1 | blaNDM-1 | p18-43_01-like | |||
| 32 | 9501453777 | S. marcescens | NA | 1 | blaNDM-1 | p18-43_01-like | |||
| 33 | 945154301 | S. marcescens | NA | 2 | blaNDM-1 | p18-43_01-like | |||
| 34 | 945174350 | S. marcescens | NA | 2 | blaNDM-1 | p18-43_01-like | |||
| 36 | 19870317 | E. asburiae | ST108 | blaNDM-1 | p18-43_01-like | ||||
| 37 | 939705067 | E. asburiae | ST435 | blaNDM-1 | p18-43_01-like | ||||
| 40 | 950180354 | E. cloacae complex | ST145 | blaNDM-1 | p18-43_01-like | ||||
| 41 | 953102574 | E. cloacae complex | ST433 | blaNDM-1 | p18-43_01-like | ||||
| 42 | 941713674 | E. kobei | ST54 | blaNDM-1 | p18-43_01-like | ||||
| 43 | 953099839 | E. kobei | ST434 | blaNDM-1 | p18-43_01-like | ||||
| 44 | 950178628 | Enterobacter spp. | ST121 | blaNDM-1 | p18-43_01-like | ||||
| 45 | 944526466 | C. freundii | ST63 | blaNDM-1 | p18-43_01-like | ||||
a
Phylogenetic subgroup as determined for K. pneumoniae and S. marcescens isolates.
b
Referred to as “-like” when plasmid sequence is not circularized but the carbapenemase-encoding contig revealed 100% nucleotide identity to the given plasmid.
c
NA, MLST is not available for this species.