TABLE 1.

Genes and primers used for qPCR in this study

Gene	Primer sequence (5′–3′)		Forward primer			Reverse primer			Product size (bp)	Reference
	Forward	Reverse	BPa	GCb	TMc	BP	GC	TM
lasI	TGTTCAAGGAGCGCAAAGG	ATGGCGAAACGGCTGAGTT	19	52.6	62.4	19	52.6	63	244	58
lasR	AGCGACCTTGGATTCTCGAAG	CGAAGAACTCGTGCTGCTTTC	21	52.4	63	21	52.4	62.5	226	58
rhlI	TGCTCTCTGAATCGCTGGAA	GTTTGCGGATGGTCGAACTG	20	50	59.1	20	55	59.83	154	58
rhlR	TTGCTGAGCGTGCTTTCC	AGGATGATGGCGATTTCCC	19	52.6	62.6	19	52.6	62.1	228	58
rpsLd	CCTCGTACATCGGTGGTGAAG	CCCTGCTTACGGTCTTTGACAC	21	57.1	62.8	22	54.5	63.1	148	59
proDd	GGGCGAAGAAGGAAATGGTC	CAGGTGGCGTAGGTAGAGAA	20	55	63.1	20	55	58	178	60

^aBP, primer length (base pairs).

^bGC, G-C content of primer.

^cTM, melting temperature of primer.

^dReference/endogenous control gene.