FIG 2.

(A) Sequence analyses of the mcr-1 promoter region. Red shading denotes identical residues, yellow shading shows ISApl1 or partial ISApl1 sequences, and blue shading indicates IS5 sequences. The putative −35 and −10 promoter sequences in pGZ49266 and pGZ49273 are shown in dashed boxes. The cloning primer sequences of Pmcr-1_m, pGZ49260/pGZ49271, pGZ49266, and pGZ49273 are shown as underlining arrows. The primer sequences of pGZ49263 and pGZ49269 are located upstream of this region. S, transcription start site; M, methionine and translation initiation site. (B). Levels of mcr-1 gene expression in E. coli DH5α strains with different plasmid promoter sequence constructs (pQF50). The MIC values for colistin and polymyxin B are shown above the graph.