TABLE 1.
Susceptibility of HGT-NV cells to rupintrivir after repetitive cultivation
| Rupintrivir concn (μM) at final passage or control treatment | Clone no. | Mean EC50 ± SDa (μM) | Fold change in sensitivityb | Amino acid substitution(s) in protease |
|---|---|---|---|---|
| 20 | 1 | 14.3 ± 1.7 | 10.9 | A105V/I109V |
| 2 | 12.3 ± 1.2 | 9.4 | A105V/I109V | |
| 3 | 15.3 ± 1.1 | 11.7 | A105V/I109V | |
| 15 | 1 | 8.5 ± 1.9 | 6.5 | A105V/I109V |
| 2 | 14.3 ± 1.3 | 10.9 | A105V/I109V | |
| 3 | 6.9 ± 1.7 | 5.3 | I109V | |
| 10 | 1 | 6.6 ± 2.4 | 5.0 | I109V |
| 2 | 6.3 ± 2.3 | 4.8 | I109V | |
| 3 | 5.9 ± 1.3 | 4.5 | I109V | |
| DMSO | 1.3 ± 0.2 | 1.0 |
a
The EC50s are the means ± standard deviations from three independent experiments.
b
Fold change in sensitivity relative to that achieved with DMSO.