TABLE 1.
Effect of ANT431 on inhibition of purified MBLs and potentiation of MEM activity against E. coli BL21(DE3) transformed with pET plasmids containing NDM-1, VIM-1, VIM-2, or IMP-1
| Compound | NDM-1 |
VIM-1 |
VIM-2 |
IMP-1 |
||||
|---|---|---|---|---|---|---|---|---|
| Kia | MICb | Ki | MIC | Ki | MIC | Ki | MIC | |
| None | 32 | 4 | 4 | 8 | ||||
| ANT431 | 0.29 | 0.25 | 14.6c | 4 | 0.19 | 0.06 | 4.15 | 4 |
a
Ki, enzyme inhibition constant (micromolar).
b
MIC of MEM (micrograms per milliliter) determined alone or in the presence of 30 μg/ml of compound.
c
Determined from Dixon plot analysis (Fig. 2).