TABLE 3.
MICs for recombinant Aspergillus fumigatus strains with different Cyp51A amino acid substitutions according to the EUCAST method
| Isolate | Cyp51A substitution(s) |
EUCAST MIC (mg/liter)a |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRb | Coding gene | ITC | VRC | POS | EPO | BRO | TEB | DIF | PRO | IMA | PRC | |
| CEA Δku80 recipient strain | − | − | 0.5 | 0.5 | 0.125 | 2–4 | 2 | 2 | 1–2 | 4 | 0.125 | 0.25 |
| CEA Δku80_S297T | − | S297T | 0.5–1 | 1 | 0.25 | 4 | 4 | 2 | 2 | 4 | 0.25 | 0.5 |
| CEA Δku80_F495I | − | F495I | 0.5 | 0.5–2 | 0.03–0.125 | 2–4 | 1 | 1–2 | 2 | 8 | 0.5 | 0.5–1 |
| CEA Δku80_TR34/L98H | + | L98H | ≥16 | 4 | 1 | ≥32 | 16 | 16–32 | 16 | ≥32 | 4–8 | 2 |
| CEA Δku80_TR34/L98H/S297T | + | L98H/S297T | 8–16 | 2 | 0.5–1 | ≥32 | 32 | 16 | 16 | ≥32 | 2–4 | 4 |
| CEA Δku80_TR34/L98H/F495I | + | L98H/F495I | ≥16 | 2 | 0.5 | ≥32 | 8 | 16–32 | ≥32 | ≥32 | 8 | >32 |
| CEA Δku80_TR34/L98H/S297T/F495I | + | L98H/S297T/F495I | ≥16 | 1–2 | 1 | ≥32 | ≥32 | 16 | ≥32 | ≥32 | 16 | ≥32 |
a
ITC, itraconazole; VRC, voriconazole; POS, posaconazole; EPO, epoxiconazole; BRO, bromuconazole; TEB, tebuconazole; DIF, difenoconazole; PRO, propiconazole; IMA, imazalil; PRC, prochloraz.
b
TR, 34-bp tandem repeat in the promoter region. −, absent; +, present.