FIG 5.

Effects of ZAC307 and ZAC989 on the growth capacity of several A. fumigatus strains. (A) The wild-type AF14 strain was cultured in 24-well culture plates inoculated with 10⁵ conidia per well in a total volume of culture medium of 1 ml. Culture media that were not inoculated (Ni) were used as background reference. Media were not supplemented with zinc or were supplemented with 2, 5, or 50 μM ZnSO₄ in the absence (−) or the presence (+) of ZAC307 (Z7) or ZAC989 (Z9), as indicated. Plates were incubated at 37°C in a humid atmosphere for 44 h, scanned, and quantified, and the growth was represented graphically. (B) Effect of ZACs on the growth capacity of the ΔzrfA ΔzrfB mutant strain (AF48). (C) Effect of ZACs on the growth capacity of the ΔzrfA ΔzrfB ΔzrfC mutant strain (AF721). (D) Effect of ZACs on the growth capacity of the ΔzrfA ΔzrfB ΔzrfC (zrfC) mutant strain (AF731). (E) Effect of ZACs on the growth capacity of the ΔzrfC mutant strain (AF54). The AF48, AF721, AF731, and AF54 strains were all cultured and incubated in 24-well culture plates, and their growth was quantified as described for the wild-type strain. In all cases the relative arbitrary units obtained after quantification of the plates were normalized by taking the average of the background values of cultures which were not inoculated as a growth capacity of 0% and the growth reached by the wild-type strain in media supplemented with 50 μM zinc in the absence of ZACs as a growth capacity of 100%. In all graphs, the data represent the averages and SDs from two independent experiments in which all strains had been cultivated in duplicate.