Fig. 3.

HPV-16 E7 expression confers resistance to the antiproliferative effect of rapamycin. a DNA synthesis in organotypic cultures of keratinocytes was detected by BrdU incorporation followed by immunohistochemistry. Arrows indicate BrdU-positive nuclei. b The percentage of BrdU-positive/total nuclei was determined by direct nuclei counting. The bars correspond to values obtained in at least three independent different experiments. (*) p < 0.05. c Growth curve of monolayer cultures of keratinocytes treated with 100 ng/ml rapamycin for 7 days. Results are representative of three independent experiments performed in triplicates. (*) p < 0.05; (**) p < 0.001. d Rapamycin treatment is associated with increased PLD activity in cultures of PHK expressing HPV16 E7. PLD activity was determined as described in Fig. 2b. Scale bars, 20 μm