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. 2018 Apr 27;8:6639. doi: 10.1038/s41598-018-25094-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of Il31^−/− mice. (a) IL-31 gene targeting strategy. The region containing a part of exon 2 and exon 3 of Il31 was replaced with a cassette consisting of IRES-EGFP and a neomycin resistance gene (Neo^r), flanked by loxP sequences. (b) Expression of Il31 mRNA in lung tissue from wild-type (n = 8) and Il31^−/− (n = 8) mice by qPCR. The data show the mean + SEM. (c) Leukocyte profiles in the spleen from wild-type and Il31^−/− mice (n = 3). The percentages of CD19⁺ CD3⁻ and CD19⁻ CD3⁺ cells and of Gr1⁺ Siglec F⁻ and Gr1^- Siglec F⁺ cells in 7-AAD-negative spleen cells, and the percentages of CD8⁺ CD4⁻ and CD8⁻ CD4⁺ cells in 7-AAD-negative CD3⁺ spleen cells are shown. Representative flow cytometry data are shown.