Table III.
Permeability of water across (Pf in micromoles per second) different membrane vesicles isolated from squash root using a stopped-flow device and inhibition by 0.5 mm of HgCl2
| Type of Membrane Vesicle Used | Control | HgCl2 | HgCl2+ 2-Mercaptoethanol |
|---|---|---|---|
| Microsomal vesicles | 36.9 ± 4.7a | 11.7 ± 3.1 (68%)a | 28.9 ± 8.5 (21%) |
| Plasma membrane vesicles | 23.9 ± 3 | 20.6 ± 5 (14%) | 23.4 ± 3.6 (2%) |
| Plasma membrane-depleted vesicles | 53.9 ± 1.1a | 31 ± 5.8 (42%)a | 51.9 ± 3.1 (4%) |
The percent of inhibition in the presence of 0.5 mm HgCl2 is shown in parentheses. The experiment was repeated three times and six to eight replicates were used in every treatment. Statistical significance at the 5% level according to the Student's t test.
a
Statistically significant difference between the control and the HgCl2 treatment.