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. 2018 Apr 27;8:6640. doi: 10.1038/s41598-018-24622-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative image of mouse heart DTI. (A) Zoomed-in view of 3D diffusion tensors calculated at each voxel in the short-axis plane. Orientation of the anisotropy of the diffusion tensor is conventionally assumed to be parallel to the underlying cardiomyocytes orientation. Diffusion tensors are color-coded based on the calculated helix angle. (B) Zoomed-in view of 3D cardiomyocytes tracking in the myocardium. (C) Reconstructed cardiomyocytes above the mid-left ventricle on a short-axis plane. (D) 3D tractography of the helix angle of a mouse heart visualizes the helical structure of the cardiomyocytes twisting around the left ventricle.