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. 2018 Apr 27;8:6640. doi: 10.1038/s41598-018-24622-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative helix angle maps of control and ischemic mouse hearts from optical imaging and DTI. (A) Helix angle transmurality of the control and ischemic groups revealed significant reduction in overall helical structure in diseased tissue (DTI: 0.79 ± 0.13°/% transmural depth [TD] and optical imaging: 0.84 ± 0.26°/%TD), compared with control tissue (DTI: 1.31 ± 0.20°/% TD and optical imaging: 1.36 ± 0.27°/%TD), specifically in the endocardium (all p < 0.001). (B) Linear correlation analysis demonstrating significant correlation (R² = 0.803) between DTI and optical images. (C) Bland-Altman plot demonstrating similarity between the optical imaging-based and DTI-based cardiomyocytes helix angle transmurality estimation (Bland-Altman limits of agreement, −0.33 to 0.24°/% transmural depth [TD]).