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. 2018 Apr 27;8:6661. doi: 10.1038/s41598-018-25049-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence profile of EGFP in CHO-S cells. CHO-S cells transfected with vectors containing IRES elements from different resources were measured using FACS after 48 h transfection. (A) Representative of high EGFP fluorescence profile in cells with the pIRES-EGFP-9. (B) Representative of medium EGFP fluorescence profile in cells with the pIRES-EGFP-4. (C) Representative of low EGFP fluorescence profile in cells with the pIRES-EGFP-2. (D) Representative of untransfected cells. Scale bar, 100 μm.