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. 2018 Apr 27;8:6661. doi: 10.1038/s41598-018-25049-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative positive colony in stably transfected CHO-S cells. Transfection and drug selection were carried out as stated in the portion of materials and methods. Single clones growing from each of the population were photographed with fluorescence microscope. Three stably single clone pools were chosen for vectors. EGFP fluorescence in cells stably transfected with pIRES-EGFP-9 (A) pIRES-EGFP-8 (B) and pIRES-EGFP-2 (C). (D) Image of colony under white light. Scale bar, 50 μm.