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. 2018 Apr 27;9:1696. doi: 10.1038/s41467-018-04121-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Structural determinants of TAK1 required for αTAT1 binding. a Schematic representation of TAK1 WT and C-terminal truncated mutant TAK1-ΔCT. b COS-7 cells expressing HA-αTAT1 WT, Flag-TAK1, or Flag- TAK1-ΔCT were immunoprecipitated with Flag antibody. c COS-7 cells expressing HA-αTAT1 WT, Flag-TAK1, or Flag-TAK1-ΔCT were immunoprecipitated with αHA antibody. d COS-7 cells expressing Flag-TAK1 WT, Flag-TAK1 KD (K63W), and Flag-TAK1-ΔCT were fixed and imaged for Flag (left panel) and Acetyl-tubulin (right panel) immunofluorescence co-staining. CTCF was quantified using Image J, at least 30 cells were quantified per condition. The images are representative of three independent experiments, n = 3. Error bars represent SEM and type 2 t-test analysis show relative to control: *not significant; **p < 0.001. e Schematic representation of αTAT1-WT and truncation mutants αTAT1-Δ301, αTAT1-Δ196. f COS-7 cells expressing Flag-TAK1 WT, HA-αTAT1 WT, HA-αTAT1-Δ196, or HA-αTAT1-Δ301 were immunoprecipitated with α-HA antibody. g, h COS-7 cells expressing HA-αTAT1 WT, HA-αTAT1-Δ196, or HA-αTAT1-Δ301 were fixed and imaged for α-HA (left panel) and Acetyl-tubulin (right panel) immunofluorescence co-staining. CTCF was quantified using Image J, at least 30 cells were quantified per condition. The images are representative of three independent experiments, n = 3. Error bars represent SEM and type 2 t-test analysis show relative to control: *not significant; **p < 0.001