Fig. 7.

TAK1-dependent αTAT1 activity in the brain. a Western blot analyses demonstrating phosphorylation levels on serine-237 residue of αTAT1 in lysates extracted from mouse tissues such as forebrain, periaqueductal gray (PAG), liver, pancreas, heart, lung, and spleen. b Representative western blottings demonstrating phospho-αTAT1-Ser237 and total αTAT1 levels in lysates extracted from mice (n = 4) with and without treatment with TAK1 inhibitor 5Z-7-oxozeaenol (20 µg or 40 µg per animal; 1 h). Band density of four independent blots was calculated using ImageJ. Error bars represent SEM and type 2 t-test analysis show relative to control: *p < 0.05. c Representative western blottings demonstrating acetyl tubulin, total tubulin, pGSK3β, cMYC, and pAKT-S473 levels in lysates extracted from mice (n = 4) with and without treatment with TAK1 inhibitor 5Z-7-oxozeaenol (20 µg or 40 µg per animal; 1 h). Band density of four independent blots was calculated using ImageJ. Error bars represent SEM and type 2 t-test analysis show relative to control: *p < 0.05 (pAKT), **p < 0.05 (cMYC), ***p < 0.05 (acetyl tubulin), NS is not statistically significant (pGSK3β). d Immunofluorescence images demonstrating acetyl tubulin (green) levels in neurons (red, Nissl stain) extracted from the dorsal root ganglia (DRG) of mice. The neurons were subjected to TAK1 inhibitor 5Z-7-oxozeaenol (20 µM; 1 h), 24 h post dissection. CTCF per unit area was quantified using Image J, at least 40 cells were quantified per condition. Error bars represent SEM and type 2 t-test analysis show relative to control: *p < 0.05