Table 1. Composition of the tested buffers.
| Composition | Buffer 1a | Buffer 2b | Buffer 3c |
|---|---|---|---|
| Buffering agent | Tris 25 mM | Tris 25 mM | Tris 25 mM |
| Surfactant | CHAPS 2% | CHAPS4% TRITON 1% | CHAPS 4% |
| Protease inhibitors | Aprotinine, leupeptine, pepstatine, benzamidine and PMSF | Aprotinine, leupeptine, pepstatine, benzamidine and PMSF | Aprotinine, leupeptine, pepstatine, benzamidine and PMSF |
| Chaotropes | 7 M Urea | ||
| 2 M Thiourea | |||
| Reducer | 65 mM DTT |
Notes.
a
Buffer 1 with power low solubility (few surfactants without chaotropes).
b
Buffer 2 with median solubilizing power (more surfactants).
c
Buffer 3 with maximum solubilization power (presence of chaotropes, surfactants and reducers).