Fig. 4.

Ebselen efficiently oxidizes SOD1 in living cells. a Overlay of ¹H-¹⁵N NMR spectra acquired on human cells expressing [U-¹⁵N] labelled wild-type E,Zn-SOD1 in the absence (red) and in the presence (black) of ebselen in the external medium. b Overlay of ¹H-¹⁵N NMR spectra acquired on purified, disulphide-reduced [U-¹⁵N] labelled wild-type E,Zn-SOD1 before (blue) and after (magenta) treatment with 1 equivalent of ebselen. The positions of the enlarged areas (bottom panels) in the whole spectra (top panels) are indicated as dotted lines. Signals affected by the formation of the C57–C146 disulphide bond are labelled in the bottom panels