Fig. 4.

Frequency and distribution of high frequency variants across replicated populations for 143 variable genomic positions. Only high frequency variants (variants that evolved de novo, reached a frequency of 50% in at least one population, and the position had sufficient coverage in 6 out of 10 clones; see Results and Methods sections for more details) are plotted, with their corresponding frequency in the other populations. Each column represents a particular variable genomic position and positions were ordered to best visualize population specific and shared variants across populations. White color indicates no single clone out of 10 of this population had a variant at this position, yellow to red colors indicate increasing frequencies of the variant in that population. Total number of variants = 143. Abbreviations coe1–coe3 correspond to the three coevolved populations. Abbreviations evo1–evo3 correspond to the three evolved populations