Figure 6.

Labeling of sulfenylated proteins in mitochondria under increased oxidative conditions. (a) The effect of increased oxidant levels on DCP-NEt₂C labeling in A549 cells. Compared to no probe or no ROS: NS = effect size small, **p < 0.005, effect size large. (b) The effect of increased oxidant levels on DCP-Rho1 labeling in A549 cells. Compared to no probe or no ROS: **p < 0.005, effect size large. (c) The effect of fetal bovine serum (FBS) deprivation on DCP-NEt₂C (50 µM) labeling in A549 cells. Compared to no probe or no ROS: **p < 0.005, effect size large, ***p < 0.005, effect size very large. (d) The effect of fetal bovine serum (FBS) deprivation on DCP-Rho1 (10 µM) labeling in A549 cells. Compared to no probe or no ROS: ***p < 0.005, effect size very large. (e) Decrease in mitochondrial membrane potential caused by AgNPs and irradiation measured by TMRE: compared to no exposure, **p < 0.0005, effect size large. (f) Labeling of sulfenylated protein by DCP-NEt₂C in cells exposed to AgNPs and irradiation: compared to no exposure, NS p < 0.0005, effect size small, *p < 0.0005, effect size medium, ***p < 0.0005, effect size very large. (g) Labeling of sulfenylated protein by DCP-Rho1 in cells exposed to AgNPs and irradiation: compared to no exposure, NS^ p = 0.06, effect size very small, NS p < 0.0005, effect size small, *p < 0.0005, effect size medium, **p < 0.0005, effect size large.