Fig. 4. C2EIP promotes PGC generation in vivo.

a Gene expression of CVH, and CKIT was quantified by qRT-PCR. b Protein expression of CVH, and C-KIT was assessed by western blot analysis. Chicken embryos without any treatment were used as controls. β-actin was used as an internal reference. c Immunofluorescence of the genital ridge in control and C2EIP overexpression (OE) and knockout (KO) hatching chicken embryos. Chicken embryos without any treatment were used as controls, Scale bar: 40 μm. d CKIT⁺CVH⁺ cells were quantified using flow cytometry in the indicated groups. Chicken embryos without any treatment were used as controls