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. 2018 Apr 27;9(5):500. doi: 10.1038/s41419-018-0524-y

Fig. 7. Necroptosis induced by forced dimerization of MLKL is much less effective in inducing cytokine production than that by TSZ.

Fig. 7

a Schematic representation of the oligomerizable MLKL (acMLKL) (upper) and western blotting analysis of lysates from HT-29 cells stimulated with DMSO or AP20187 for 2 h (lower). FL, full length; endo., endogenous. b HT-29 cells stably expressing the indicated constructs were stimulated with DMSO or AP20187 for 2 h. The cell viability was determined by CellTiter-Glo. c HT-29 cells expressing acMLKL were treated with the indicated compounds for 6 h except AP20187, which was administered in the last 2 h. The cell viability was determined by CellTiter-Glo. d HT-29 cells stably expressing the vector or acMLKL were stimulated with DMSO or AP20187 for 2 h. Cxcl8 mRNA levels were measured by qPCR. e HT-29 cells stably expressing acMLKL were stimulated with AP20187 for the indicated time. The expression of Cxcl8 was measured by qPCR (left). Cell viability was determined by CellTiter-Glo (right). f The acMLKL expressing HT-29 cells were treated with the indicated compounds for 6 h except AP20187, which was added during the last 2 h of incubation. The expression of Cxcl8 was measured by qPCR. g HT-29 cells stably expressing acMLKL were treated as indicated for 8 h except AP20187. AP20187 was administered in the last 2 h. The expression of Cxcl8 was measured by qPCR. The cell viability was determined by CellTiter-Glo. Data were represented as mean ± SEM of triplicates