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. 2018 Apr 27;8:6685. doi: 10.1038/s41598-018-25034-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fecal P. aeruginosa loads over time following peroral infection of mice suffering from chronic colitis. Conventionally colonized IL-10^−/− mice with chronic colitis (black circles) were perorally infected with a multidrug-resistant P. aeruginosa strain on day (d) 0. Wildtype mice without large intestinal inflammation (white circles) served as (infected) controls. (a) Intestinal colonization densities were determined in fecal samples until d28 postinfection by culture and expressed as colony forming units per gram (CFU/g). (b) Upon necropsy at day 42 postinfection, pathogenic loads were cultured from distinct compartments of the gastrointestinal tract (i.e. stomach, duodenum, ileum and colon). Numbers of mice harboring P. aeruginosa out of the total number of analyzed mice, medians (black bars) and significance levels (p-values) determined by Mann Whitney U test are indicated (*p < 0.05; ***p < 0.001). Data shown were pooled from at least three independent experiments.