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. 2018 Apr 27;8:6685. doi: 10.1038/s41598-018-25034-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intestinal microbiota composition following multidrug-resistant P. aeruginosa infection of mice suffering from chronic colitis. IL-10^−/− mice with chronic colitis (n = 19) were perorally infected with a multidrug-resistant P. aeruginosa strain on day (d) 0. Before (N, naive; white circles) and six weeks thereafter (d42 postinfection; black circles) a comprehensive survey of the intestinal microbiota composition was performed on fecal samples applying both (a) culture (expressed as colony forming units per gram, CFU/g) and (b) 16S rRNA based molecular analyses (expressed as gene numbers per ng DNA) of the total bacterial load (TL) and main intestinal bacterial groups including enterobacteria (EB), enterococci (EC), lactobacilli (LB), Bacteroides/Prevotella spp. (BP), Clostridium/Eubacterium spp. (CE), bifidobacteria (BB), Clostridium coccoides group, Clostridium leptum group (CL) and Mouse Intestinal Bacteroides (MIB). Medians are indicated. Data shown were pooled from three independent experiments.