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. 2018 Apr 27;8:6685. doi: 10.1038/s41598-018-25034-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Colonic immune cell responses in multidrug-resistant P. aeruginosa infected mice suffering from chronic colitis. IL-10^−/− mice with chronic colitis were perorally infected with a multidrug-resistant P. aeruginosa (Psae) strain on day (d) 0. Six weeks thereafter (on d42 postinfection; black circles) the average numbers of colonic (a) macrophages and monocytes (positive for F4/80), (b) T lymphocytes (positive for CD3), (c) regulatory T cells (positive for FOXP3), and (d) B lymphocytes (positive for B220) were determined from six high power fields (HPF, 400x magnification) per animal in immunohistochemically stained large intestinal paraffin sections. Naive (N) IL-10^−/− mice served as negative controls (open circles). Numbers of mice (in parentheses), medians (black bars) and significance levels (p-values) determined by the Mann Whitney U test are indicated. Data shown were pooled from three independent experiments.