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. 2018 Apr 27;8:6659. doi: 10.1038/s41598-018-25139-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intracellular accumulation of MPP⁺ and TMAO [MPP⁺ (50 µM) (a), TMAO (10 µM) (b), and TMAO (100 µM) (c)] in MDCK cells grown as polarised monolayers expressing OCT2 (MDCK-OCT2), MATE1 (MDCK-MATE1), or both OCT2 and MATE1 (MDCK-OCT2-MATE1) and control cells (MDCK-Co) after 60 min. Each data point is 2 × n = 3, mean ± SEM, ***p < 0.001 vs. MDCK-Co, ^#p < 0.05 vs. MDCK-OCT2, ^###p < 0.001 vs. MDCK-OCT2, ^§§§p < 0.001 vs. MDCK-MATE1.