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. 2018 Apr 27;8:6668. doi: 10.1038/s41598-018-25085-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AgNPs increase collagen deposition in BEAS-2B cells. Collagen production after 6 weeks of exposure (5 days after final re-seeding) was quantified using the Sircol assay. Soluble collagen secreted in the cell medium (A) was expressed as µg/mL, acid extracted collagen (insoluble collagen deposited on the well plate) (B) was expressed as µg/mL and total collagen (C) was expressed as µg. Results are presented as mean values ± S.D. (n = 3). *p < 0.05, ***p < 0.001.