Figure 3.

Expression of AtLKR/SDHp fused to N-terminal His tag in yeast and its purification. A, Protein extracts from yeast cells harboring the pVT-At-His-LKR/SDH plasmid or control vector alone (lanes a and b, respectively) were reacted in a western blot with anti-His-tag antibodies. B, Protein extracts from yeast cells harboring the pVT-At-His-LKR/SDH plasmid either before purification (lane a) or after purification on a nickel column (lane b) were fractionated on SDS polyacrylamide gel, and the proteins were stained with Coomassie Blue R-250. The position of AtLKR/SDHp is indicated on the right. The migrations of molecular mass protein markers are shown on the left.