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Determination of apparent Km values for SDH activity of AtLKR/SDHp toward its substrates. Apparent Km values were obtained by double reciprocal plots of the initial velocities of SDH with saccharopine (A and C) and NAD (B and D) as the variable substrates. Activity assays were performed either at pH 7 (A and B) or at pH 9 (C and D). The calculated apparent Km values are given in A, B, C, and D. These experiments were replicated three times with similar results.
