Table 5.
Presence or absence data for PLAs identified by Dowell et al. [7] (the first five PLAs) and the sixth PLA identified in this study. The first four individuals were the specimen used in Dowell et al. [7] and only presence/absence is indicated. The last nine individuals are C. scutulatus specimens sequenced in this study with venom type indicated and transcripts per million reads (TPM) values given when that PLA is present. The one TPM value denoted by an * had eight nonsynonymous nucleotide changes in the sequence compared to the other three C. scutulatus specimens and matched that of Crotalus viridis (Genbank accession AF403134).
| Specimen | Pla2gA1 | Pla2gB1 | Pla2gK | Pla2gA2-MTXA | Pla2gB2-MTXB | PLA-6 |
|---|---|---|---|---|---|---|
| C. atrox | + | + | + | - | - | - |
| C. atrox | + | + | + | - | - | - |
| C. adamanteus | + | + | - | - | - | - |
| C. scutulatus | + | - | - | + | + | - |
| CLP1930A | 55,212.77 | - | - | 603,261.66 | 294,142.52 | 47,256.79 |
| CLP1936A | 242,536.57 | - | - | 492,462.39 | 264,770.94 | - |
| CLP1959A | 214.27 | 101,885.11 | 6162.67 | 355,273.29 | 166,449.59 | 369,588.5 |
| CLP1961A | - | - | - | 464,139.64 | 323,205.86 | 212,285.6 |
| CLP1972A | - | - | - | 520,518.57 | 216,000.5 | 263,309.5 |
| CLP1831B | - | 370,853.15 | 13,305.52 | - | - | 600,310.86 |
| CLP1835B | 320,034.14 * | 297,244.14 | 18,761.21 | - | - | 58,732.73 |
| CLP2136B | - | 283,162.92 | 6014.01 | - | - | 709,003.82 |
| CLP2142B | - | 253,634.76 | 6285.41 | - | - | 738,317.63 |