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Schedule of experiment. Schematic of experimental design for the induction of atopic dermatitis (AD) in mice. One week after first boosting with 1% 2,4-dinitrochlorobenzene (DNCB), 1% DNCB (20 µL/ear) and Dermatophagoides farinae (DFE) (10 mg/mL, 20 µL/ear) was applied alternately to both ears once a week during 4 weeks. The bee venom phospholipase A2 (bvPLA2) (80 ng/ear) was topically applied four times a week for 3 weeks. On day 28, the mice were sacrificed for further analysis.
