Table 2.
Summary of the serum toxicokinetics of HFB1 administered iv (HFB1iv) in a single bolus of 139 nmol·kg·BW−1 in barrows (n = 5).
| HFB1−m | |||||||
| Animal | 1 | 8 | 13 | 21 | 28 | Mean | SD |
| BW (kg) | 34.9 | 35.6 | 33.5 | 34.0 | 32.0 | 34.0 | 1.4 |
| HFB1 (nmol·kg·BW−1) | 139 | 139 | 139 | 139 | 139 | 139 | 0 |
| A (nmol·L−1) | 69.5 | 297.3 | 205.2 | 122.8 | 724.1 | 283.8 | 260.8 |
| α (h−1) | 10.8 | 20.5 | 14.6 | 10.7 | 15.9 | 14.5 | 4.1 |
| B (nmol·L−1) | 8.8 | 1.0 | 1.2 | 42.2 | 48.6 | 20 | 23 |
| β (h−1) | 1.2 | 0.4 | 0.4 | 2.9 | 1.5 | 1.3 | 1.0 |
| t1/2α (h) | 0.06 | 0.03 | 0.05 | 0.07 | 0.04 | 0.05 | 0.02 |
| t1/2β (h) | 0.58 | 1.82 | 1.92 | 0.24 | 0.47 | 1.01 | 0.80 |
| Vd (L·kg·BW−1) | 8.5 | 21.3 | 22.4 | 1.8 | 1.2 | 11.0 | 10.3 |
| Cl (L·kg·BW−1·h−1) | 10.1 | 8.1 | 8.1 | 5.4 | 1.8 | 6.7 | 3.2 |
| AUC (nmol·L−1·h) | 14 | 17 | 17 | 26 | 78 | 30 | 27 |
| RSD (nmol·L−1) | 0.1 | 0.7 | 1.6 | 2.8 | 1.7 | 1.4 | 1.0 |
| r2 | 0.999 | 0.998 | 0.987 | 0.983 | 0.999 | 0.993 | 0.008 |
| HFB1+m | |||||||
| A (nmol·L−1) | 69.8 | 299.1 | 205.8 | 138.8 | 724.5 | 287.6 | 258.5 |
| α (h−1) | 11.2 | 20.6 | 14.7 | 7.4 | 15.6 | 13.9 | 4.9 |
| B (nmol·L−1) | 9.6 | 1.1 | 1.2 | 8.9 | 51.3 | 14.4 | 21.0 |
| β (h−1) | 1.2 | 0.4 | 0.3 | 0.9 | 1.5 | 0.9 | 0.5 |
| t1/2α (h) | 0.06 | 0.03 | 0.05 | 0.09 | 0.05 | 0.06 | 0.02 |
| t1/2β (h) | 0.56 | 1.81 | 2.37 | 0.80 | 0.45 | 1.20 | 0.85 |
| Vd (L·kg·BW−1) | 8.0 | 21.0 | 26.2 | 5.5 | 1.1 | 12.4 | 10.7 |
| Cl (L·kg·BW−1·h−1) | 9.9 | 8.0 | 7.7 | 4.8 | 1.7 | 6.4 | 3.2 |
| AUC (nmol·L−1·h) | 14 | 17 | 18 | 29 | 80 | 32 | 28 |
| AUCHFB1−m% (%) | 98.6 | 98.9 | 95.3 | 89.6 | 98.1 | 96.1 | 3.9 |
| RSD (nmol·L−1) | 0.1 | 0.8 | 1.6 | 3.0 | 2.0 | 1.5 | 1.1 |
| r2 | 0.999 | 0.995 | 0.987 | 0.981 | 0.998 | 0.992 | 0.008 |
BW = body weight; −m = HFB1 alone; +m = HFB1 plus metabolites: pHFB1a, pHFB1b; A, B = initial concentrations for distribution and elimination, respectively, at t = 0; α and β = rate constants of distribution or elimination phase; t1/2α and t1/2β = half-lives of distribution and elimination, respectively; Vd = apparent volume of distribution, Cl = serum clearance, AUC = area under the time vs. concentration curve; AUCHFB1−m% = AUC of HFB1 alone without metabolites compared to HFB1 plus sum of metabolites in %; RSD = residual standard deviation of the regression; r2 = coefficient of determination; SD = standard deviation. Data were evaluated with Students t-test for independent data sorted in groups (for parametric data).