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. 2018 Apr 12;10(4):154. doi: 10.3390/toxins10040154

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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BVC induced the ROS accumulation and p38/JNK activation in time-dependent manner. (A) Cells were treated with 12.5 µM BVC for 1–9 h and ROS level was detected by flow cytometer analysis. (B) Quantitative analysis of the ROS. (C) Effects of BVC on p38/p-p38 expressions in HepaRG cells. The cells were treated with 12.5 µM BVC for 1, 3, 6, and 9 h, and then the expressions of the indicated proteins were evaluated by Western blotting. (D) Quantitative analysis of the p-p38 protein expression. (E) Representative Western blotting of JNK/p-JNK expressions. (F) Quantitative analysis of the p-JNK/JNK protein expression. Significances refer to comparison to control, * p < 0.05 and ** p < 0.01.