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. 2018 Apr 12;10(4):154. doi: 10.3390/toxins10040154

Figure 4.

Figure 4

Inhibition of p38/JNK phosphorylation decreased BVC-induced ROS. (A,B) Cells were pretreated with SB203580 (a p38 inhibitor, 12.5 μM) for 2 h, followed by exposure to BVC (12.5 μM) for 1 h, and ROS were assayed by FACS. (C,D) Western blotting was used to identify its protein expression of p-p38 and p38 after BVC (12.5 μM) exposure for 15, 30, and 60 min. (E,F) Cells were pretreated with SP600125 (a JNK inhibitor, 12.5 μM) for 2 h, followed by exposure to BVC (12.5 μM) for 6 h, and ROS were assayed by FACS. (G,H) Western blotting was used to identify its protein expression of p-JNK and JNK after BVC (12.5 μM) exposure for 1, 3, and 6 h. Significances refer to comparison to control, * p < 0.05 and ** p < 0.01. # p < 0.05 and ## p < 0.01 between BVC group.