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. 2018 Apr 27;8:6688. doi: 10.1038/s41598-018-25091-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding activity of EV-R vs. EV-V in the interaction of human PSGL-1 and SCARB2. Ninety-six-well plates coated with live EV-V or EV-R (10³ pfu) were incubated with various amounts of recombinant (A) hSCARB2-Fc or (B) hPSGL-1-Fc per well for 1 hour at room temperature. After incubation, the plates were washed three times with PBS, and then the EV71 particles in the wells were quantified by incubating with MAB979 antibody in an ELISA assay as described in the Materials and Methods. Three independent experiments were performed, and one of results is shown. One tail unpaired student’s t-test was used for statistical analysis.