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. 2018 Mar 22;10(4):89. doi: 10.3390/cancers10040089

Figure 3.

Figure 3

Percentage of AGS-BX1 and HONE1-EBV cells expressing EBV lytic reactivation following treatment with curcuminoids. AGS-BX1 cells were treated with several curcuminoids at their optimal lytic concentrations for 48 h. (A) The cells expressing GFP-lytic EBV reactivation were visualized under a fluorescence microscope at 20x magnification and the percentage of lytic cells was calculated. Approximately 3% of untreated AGS-BX1 cells expressed spontaneous lytic reactivation (DMSO); (B) Representative images (63× magnification) of GFP-positive HONE1-EBV cells induced by compound EF24 compared to positive control GCb+VPA. For quantification, GFP-expressing EBV lytic cycle was classified into two category: low lytic (weak GFP expression) and high lytic (strong GFP expression); (C) The percentage of AGS-BX1 and HONE1-EBV cells induced into lytic cycle was estimated by calculating number of cells expressing low and high lytic activation. Although most cells induced by hit compounds expressed low lytic, strong GFP expressions (high lytic activation) were observed in approximately 20–22% of AGS-BX1 and HONE1-EBV cells upon EF24 treatment.