Figure 5.

Naturally infected EBV positive carcinomas were more resistant to cytotoxicity effect of curcuminoids than those of recombinant EBV carcinoma cell lines. Cytotoxicity effect of hit compounds (41, EF24) was evaluated in GC (A,C,E,G) and NPC cells (B,D,F,H)). Treatments with SAHA and GCb+VPA were included as controls. AGS (EBV-negative), AGS-BX1 (recombinant EBV) and SNU-719 (native EBV) represent GC cells whereas HONE1 (EBV-negative), HONE1-EBV (recombinant EBV) and C666.1 (native EBV) represent NPC cells. All cells were incubated with various concentrations of lytic induction agents for 72 h and cell proliferation of treated cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results are expressed as percentages of treated cells compared with those of untreated cells and data from three independent experiments are presented. Standard deviation is shown in error bars. Dot line represents IC₅₀ of compound/regimen in GC and NPC cells.