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. 2018 Mar 30;10(4):97. doi: 10.3390/cancers10040097

Figure 1.

Figure 1

Staurosporine (STS), but not Nanopulse Stimulation (NPS), induces caspase-3/7 activity in 4T1-Luc mammary cancer cells. 4T1-Luc cells were treated with 100 µM and 1 mM STS or treated with 10, 20, and 50 pulses with 60 ns pulse durations and electric fields of 30, 40, and 50 kV/cm. Control (C) cells were untreated with STS or NPS. Five (5) hours later, caspase-3/7 activity was determined using the Caspase-Glo assay (ProMega, Madison WI) according to the supplier’s recommendation. The figure represents a typical assay with triplicate determinations.