Figure 4.
Luciferase assays with targeted AAVP (RGD-Luc) and non-targeted AAVP vectors (fd-Luc) expressing a luciferase (Luc) reporter gene at two different amounts of transduction with AAVP, 5 × 105 and 1 × 106 transducing units (0.5 million (M) TU/cell and 1 million (M) TU/cell, respectively). (a) M21 cells at day 4 post vector transduction, cultured with increasing concentrations of C1A. Transgene expression was dependent on targeting and increased both with dosage of C1A and AAVP TU. (b) M21 cells at day 4 post vector transduction and cultured with sodium butyrate (NaBu). Transgene expression was dependent on targeting and increased with dosage of sodium butyrate and with increasing AAVP TU. Experiments were carried out in triplicates and repeated twice. The vertical bars indicate standard deviation.