Figure 5.
B16 cytotoxicity and luciferase assays. B16 cells were cultured in 96 well plates and treated with increasing concentrations of either C1A (a) or sodium butyrate (b). Cell viability percentages are relative to parallel control untreated cell cultures after 24 h. (c,d), luciferase assays at day 4 post transduction with targeted AAVP vector (RGD-Luc) or non-targeted AAVP (fd-Luc) expressing a Luc reporter gene at two different amounts of AAVP, 5 × 105 (0.5 million (M) TU/cell) and 1 × 106 (1 million (M) TU/cell) and increasing concentrations of either C1A (c) or sodium butyrate (d). Transgene expression was dependent on targeting and increased with dosage of adjuvant and with increasing AAVP TU. Experiments were carried out in triplicates and repeated twice. The vertical bars indicate standard deviation.