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HPLC analyses of one-step folding products of linear Lt1.3. Traces from bottom to top: (a) the linear peptide; (b) one-step oxidized products; (c) the purified product of Lt1.3-I; and (d) the purified product of Lt1.3-II. Samples were applied to a Kromasil C18 column (5 μm, 4.6 mm × 250 mm) and eluted with a linear gradient of 5–10% B for 0–1 min; 10–50% B (B is acetonitrile containing 0.1% TFA) for 1–25 min. Absorbance was monitored at 214 nm. The flow rate was 1.0 mL/min.
