Figure 3.

Rapid emergence of functional RNA motifs. (A) Inoculation of PSTVd^IntC227U mutant led to the emergence of new variants in progeny, which showed up in systemic leaves; (B) Northern blots showed the presence of PSTVd in pooled samples from systemic leaves. C-PSTVd, circular PSTVd. rRNAs served as the loading control; and (C) illustrations of loop 19* and loop 19′. The U at the 227 position in the mutant background is highlighted in red, and the newly-emerged substitutions of A or U at 134 position are highlighted in green.