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Workflow of the in vivo Stl:DNA binding assay: The p2NIL-LacZStr-int vector carrying the desired Stl binding site in the promoter region of lacZ gene was electroporated into M. smegmatis cells (Step 1). After electroporation, this vector integrates into the genome. This is followed by Step 2: electroporation of pKW08-Stl vector into the Stl switch system. Stl expression is inducible in a tetracycline-dependent way.
