Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 3;10(4):171. doi: 10.3390/v10040171

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Schematic illustration of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-mediated Epstein-Barr virus (EBV)-bacterial artificial chromosome (BAC) cloning. (A) CRISPR/Cas9 target sequence used for EBV-BAC cloning. Twenty bp sequences are shaded in yellow, and PAM sequences (5′-NGG) are shaded in green. BssHII sites are underlined. Note that the target sequence is 100% conserved among representative EBV strains so far sequenced. (B) Outline of EBV-BAC cloning. EBV latently infected cells are transfected with two circular plasmids (pX330-sgEBV and a donor plasmid). CRISPR/Cas9-mediated cleavage of EBV genome DNA stimulates homology-directed repair, and transgenes are integrated into EBV episomes via homologous recombination. EBV-BAC clones can readily be obtained by transforming DH10B E. coli with episomal DNA.