Figure 4.

Bacterial colonies harboring EBV-BAC clones are larger than those harboring donor plasmids. EBV-positive gastric cancer cell line SNU-719 was subjected to EBV-BAC cloning. Individual chloramphenicol-resistant bacterial colonies obtained by transforming E. coli with episomal DNAs were picked up and transferred to LB plates containing either chloramphenicol (12.5 μg/mL) or ampicillin (200 μg/mL). Note that E. coli colonies growing faster on a chloramphenicol plate (top) are ampicillin-sensitive (bottom) (indicated by dotted circles).