Table 1.
Characteristics of 14 Salmonella phages from the Eliava collection tested on the Typhi 10040_15_DRC_2015 isolate from the Democratic Republic of the Congo (DRC).
| Nr | Name | GenBank Accession Numbers | Source | Isolation Year | Host Strain | Family | Homology to Other Phages | Host Range (%) *All Serovars/S. Enteritidis/S. Typhimurium/S. Dublin (Total Number of Tested Isolates) | Lytic Activity on the Typhi 10040_15_DRC_2015 Isolate | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Streak Method | Spot Test | Gratia’s Method | Appelmans’ Method | |||||||||||||
| Titer (pfu/mL) | Dilution | 6 h | 18 h | 24 h | ||||||||||||
| 001 | GE_vB_N3 | ND | Mtkvari river water | 2013 | S. Enteritidis 3 | Siphoviridae | ND | 78/98/73/87 (239) | + | + | + | 109 | −1 | − | − | + |
| 108 | −2 | − | − | + | ||||||||||||
| 107 | −3 | − | + | + | ||||||||||||
| 002 | GE_vB_N5 | MG969412 | Mtkvari river water | 2013 | S. Enteritidis 3 | Siphoviridae | E. coli T5 strain ATCC 11303-B5 | 45/80/25/74 (239) | + | + | + | 108 | −1 | − | + | + |
| 107 | −2 | − | + | + | ||||||||||||
| 106 | −3 | − | + | + | ||||||||||||
| 003 | GE_vB_N8 | MG969413 | Mtkvari river water | 2013 | S. Enteritidis 3 | Siphoviridae | phage SPC35 | 65/84/67/78 (239) | + | + | − | 109 | −1 | − | − | + |
| 108 | −2 | − | − | + | ||||||||||||
| 107 | −3 | − | + | + | ||||||||||||
| 004 | GE_vB_MG | MG969411 | Tbilisi sewage water | 2013 | S. Enteritidis 3 | Myoviridae | S. phage PVP-SE1 | 47/49/59/43 (239) | − | − | + | 109 | −1 | − | − | − |
| 108 | −2 | − | − | − | ||||||||||||
| 107 | −3 | − | − | − | ||||||||||||
| 005 | GE_vB_BS | MG969407 | Black Sea water | 2013 | S. Typhimurium 4 | Myoviridae | S SPT-1, partial genome | 81/96/93/83 (239) | + | + | + | 1010 | −1 | + | − | − |
| 109 | −2 | + | − | − | ||||||||||||
| 108 | −3 | + | − | − | ||||||||||||
| 006 | GE_vB_B1 | MG969405 | Mtkvari river water | 2013 | S. Typhimurium 6 | Myoviridae | S. phage Mushroom | 80/93/83/87 (239) | + | + | + | 109 | −1 | + | − | − |
| 108 | −2 | + | − | − | ||||||||||||
| 107 | −3 | + | − | − | ||||||||||||
| 007 | GE_vB_B3 | MG969406 | Mtkvari river water | 2013 | S. Typhimurium 6 | Myoviridae | S. phage Mushroom | 81/98/73/87 (239) | + | + | + | 109 | −1 | + | − | − |
| 108 | −2 | + | − | − | ||||||||||||
| 107 | −3 | + | − | − | ||||||||||||
| 008 | GE_vB_NS7 | MG969414 | Raw cow milk | 2015 | S. Typhimurium 6 | Myoviridae | S. phage Mushroom | 75/91/81/78 (239) | + | + | + | 109 | −1 | + | − | − |
| 108 | −2 | − | + | − | ||||||||||||
| 107 | −3 | − | + | + | ||||||||||||
| 009 | GE_vB_M4 | MG969409 | Black Sea water | 2016 | S. Enteritidis 232 | Siphoviridae | S. phage vB_SenS-Ent3 | 23/64/18/22 (218) | + | + | + | 1010 | −1 | + | − | − |
| 109 | −2 | + | − | − | ||||||||||||
| 108 | −3 | − | − | − | ||||||||||||
| 010 | GE_vB_M5 | MG969410 | Black Sea water | 2016 | S. Enteritidis 407 | Siphoviridae | S. phage vB_SenS-Ent3 | 33/66/26/61 (218) | + | + | + | 108 | −1 | + | − | − |
| 107 | −2 | + | − | − | ||||||||||||
| 106 | −3 | + | − | − | ||||||||||||
| 011 | GE_vB_TR | MG969415 | Mtkvari river water | 2017 | S. Typhimurium 641 | Podoviridae | S. phage BTP1 | 40/90/28/59 (141) | − | − | + | 109 | −1 | − | − | − |
| 108 | −2 | − | − | − | ||||||||||||
| 107 | −3 | − | − | − | ||||||||||||
| 012 | GE_vB_HIL | MG969408 | Mtkvari river water | 2017 | S. Enteritidis 765 | Siphoviridae | S. phage vB_SenS-Ent3 | 58/81/75/77 (141) | + | + | − | 1010 | −1 | + | − | − |
| 109 | −2 | − | − | − | ||||||||||||
| 108 | −3 | − | + | + | ||||||||||||
| 013 | GE_vB_7A | MG969404 | Mtkvari river water | 2017 | S. Typhimurium 1328 | Myoviridae | S. phage BPS15Q2 | 37/62/28/23 (141) | + | + | + | 108 | −1 | − | − | − |
| 107 | −2 | − | − | − | ||||||||||||
| 106 | −3 | − | − | − | ||||||||||||
| 014 | GE_vB_M1 | ND | Black Sea water | 2016 | S. Enteritidis 104 | Podoviridae | ND | 12/20/19/0 (77) | + | + | − | 109 | −1 | − | − | − |
| 108 | −2 | − | − | − | ||||||||||||
| 107 | −3 | − | − | − | ||||||||||||
* The host range of the phages was determined for the total Salmonella strain collection (all serovars), with the total number of strains indicated between brackets, and for the three main serovars (S. Enteritidis/S. Typhimurium/S. Dublin) separately. ND, not done; “+”, phage lytic activity; “−”, no phage lytic activity; S. phage: Salmonella phage.