Table 2.
Evaluation of the mass spectral quality in terms of signal reproducibility (peaks detected in 100% or 70–99% of mass spectra acquired for each sample) and signal-to-noise ratio of the most intense peak when using five different isolation methods and two matrix-assisted laser desorption ionization (MALDI) matrices.
| Phage | Isolation Method | MALDI Matrix | One Term | Three Terms | |||
|---|---|---|---|---|---|---|---|
| 100% Peaks | 70–99% Peaks | Maximal Signal-to-Noise | 100% Peaks | 70–99% Peaks | |||
| 3A | CsCl gradient | FerA | 20 | 22 | 504 ± 141 | 12 | 18 |
| CsCl gradient | HCCA | 3 | 2 | 107 ± 41 | - | - | |
| FPLC | FerA | 4 | 5 | 34 ± 11 | - | - | |
| Ultrafiltration | FerA | 1 | 8 | 16 ± 4 | - | - | |
| Pellet | FerA | 23 | 9 | 517 ± 219 | 8 | 8 | |
| Pellet | HCCA | 15 | 4 | 44 ± 4 | - | ||
| 71 | CsCl gradient | FerA | 8 | 13 | 397 ± 307 | 3 | 15 |
| CsCl gradient | HCCA | 2 | 2 | 5 ± 1 | - | - | |
| FPLC | FerA | 5 | 3 | 157 ± 72 | - | - | |
| Ultrafiltration | FerA | 3 | 6 | 17 ± 1 | - | - | |
| Pellet | FerA | 11 | 11 | 557 ± 108 | 9 | 4 | |
| Pellet | HCCA | 14 | 9 | 10 ± 1 | - | - | |
| 77 | CsCl gradient | FerA | 10 | 21 | 591 ± 96 | 6 | 20 |
| CsCl gradient | HCCA | 11 | 3 | 64 ± 11 | - | - | |
| FPLC | FerA | 0 | 0 | N/A | - | - | |
| Ultrafiltration | FerA | 6 | 3 | 19 ± 7 | - | - | |
| Pellet | FerA | 12 | 9 | 247 ± 36 | 8 | 11 | |
| Pellet | HCCA | 9 | 6 | 15 ± 7 | - | - | |
| K1/420 | CsCl gradient | FerA | 41 | 13 | 398 ± 122 | 21 | 12 |
| CsCl gradient | HCCA | 17 | 3 | 429 ± 30 | - | - | |
| FPLC | FerA | 0 | 0 | N/A | - | - | |
| Ultrafiltration | FerA | 0 | 0 | N/A | - | - | |
| Pellet | FerA | 17 | 7 | 125 ± 23 | 8 | 7 | |
| Pellet | HCCA | 5 | 5 | 29 ± 3 | - | - | |
| P68 | CsCl gradient | FerA | 13 | 14 | 452 ± 122 | 7 | 9 |
| CsCl gradient | HCCA | 4 | 5 | 281 ± 18 | - | - | |
| FPLC | FerA | 19 | 11 | 227 ± 23 | - | - | |
| Ultrafiltration | FerA | 5 | 1 | 41 ± 15 | - | - | |
| Pellet | FerA | 9 | 9 | 248 ± 19 | 5 | 4 | |
| Pellet | HCCA | 4 | 4 | 399 ± 166 | - | - | |
Legend: N/A, not applicable; -, not analysed.