Skip to main content
. 2018 Apr 22;10(4):213. doi: 10.3390/v10040213

Figure 8.

Figure 8

Quantification of DENV-2 derived piRNAs (vpiRNA) by stem-loop RT-PCR. (A) quantification of vpiRNA expression level in DENV-2 infected adult females; (B) quantification of vpiRNA expression level in midguts from DENV-2 infected adult females; (C) The PCR products were run on 3% agarose gel in 1X TBE stained with ethidium bromide. Note: vpiRNA2191731, vpiRNA2191732, vpiRNA0705792, vpiRNA0705793, vpiRNA3130226 and vpiRNA0705787 differed by only a few nucleotide base lengths at the 5′ and 3′ ends. A similar pattern was also found in vpiRNA0676933 and vpiRNA1747127. **: p < 0.01; ***: p < 0.001.